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Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour
The Rat (MUC2) Mucin-2 ELISA Kit measures Mucin-2 in Rat samples
Then a biotinylated detection antibody specific for TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Rat AMN (Amnionless) ELISA Kit is a Sandwich enzyme immunoassay
Rat RUNX2 (Runt-related Transcription Factor 2) ELISA Kit Packaging:96 wells Horseradish peroxidase (HRP) bound inThe Rat (RUNX2) Runt related Transcription Factor 2 ELISA Kit measures Runt related transcription factor 2 in Rat samples. The plate has been pre coated with Rat RUNX2 antibody. RUNX2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat RUNX2 Antibody is added and binds to RUNX2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RUNX2 antibody. After incubation unbound
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