Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells When the sample containing IL-5

When the sample containing IL-5 is added to the plate

a horseradish peroxidase conjugated mouse anti-Human SAA4 Antibody is added to the wells and binds to SAA4 in the sample

Then a biotinylated detection antibody specific for Rat BetaTG/PBP/CXCL7/NAP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Mouse TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Mouse RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini-Sample) Packaging:96 wells When the sample containing IL-5This Mouse RANL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to RANL . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANL and Avidin Horseradish Peroxidase (HRP) conjugate are added