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Then Streptavidin-HRP is added and binds to the Biotinylated PIM1 antibody
Calculate the concentration of Hmgb1 in the sample by plotting standard curve
The concentration of Human sVEGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve
a capture antibody-ISM1-biotin-detection antibody complex was formed
Rat AREG (Amphiregulin) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat AREG (Amphiregulin) ELISA Kit measures AREG in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti AREG antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
