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The absorbance is measured on a microplate reader at 450 nm
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELA2A
Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G1B antibody
Mouse SYP (Synaptophysin) ELISA Kit SCYB4 The absorbance is measured onThe Mouse (SYP) Synaptophysin ELISA Kit measures Synaptophysin in Mouse samples. The plate has been pre coated with Mouse SYP antibody. SYP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SYP Antibody is added and binds to SYP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
