Human FLOT1 (Flotillin-1) ELISA Kit PADGEM Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRF

The OD value is proportional to the concentration of Human mTOR

Substrate solution is then added and color develops in proportion to the amount of Human CD247

Human FLOT1 (Flotillin-1) ELISA Kit PADGEM Then a biotinylated detection antibodyThe Human (FLOT1) Flotillin 1 ELISA Kit measures Flotillin 1 in samples. The plate has been pre coated with Human FLOT1 antibody. FLOT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FLOT1 Antibody is added and binds to FLOT1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FLOT1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.