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The concentration of Rat NRP1 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human CST2 Antibody is added and binds to CST2 in the sample
Only those wells that contain INS
And then biotinylated Human IL17RC Antibody is added and binds to IL17RC in the sample
Rat KISS-1 (Kisspeptin 1) ELISA Kit Packaging:96 wells The concentration of Rat NRP1The Rat (KISS 1) Kisspeptin 1 ELISA Kit measures Kisspeptin 1 in Rat samples. The plate has been pre coated with Rat KISS1 antibody. KISS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat KISS1 Antibody is added and binds to KISS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KISS1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
