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Chicken Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg
Then Streptavidin-HRP is added and binds to the Biotinylated CES1 antibody
The KRT12 (Keratin 12) ELISA Kit is a Sandwich enzyme immunoassay
Substrate solution is then added and color develops in proportion to the amount of Mouse WNT8A
Human PROM1 (Prominin-1) ELISA Kit Packaging:96 wells Chicken Pg in samples (orThe Human (PROM1) Prominin 1 ELISA Kit measures Prominin 1 in samples. The plate has been pre coated with Human PROM1 antibody. PROM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PROM1 Antibody is added and binds to PROM1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PROM1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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