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Calculate the concentration of PINP in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Porcine FAK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of RPIA in the sample
Calculate the concentration of SNAP-25 in the sample by plotting standard curve
Human PLA2P (Phospholipase A2 Activating Protein) ELISA Kit A1A Calculate the concentration of PINPThe Human PLA2P (Phospholipase A2 Activating Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human PLA2P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLA2P and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
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