Guinea Pig IgG (Immunoglobulin G) ELISA Kit Packaging:96 wells The OD value is proportional

The OD value is proportional to the concentration of Human PPY

Then Streptavidin-HRP is added and binds to the Biotinylated IFN-beta antibody

Then a biotinylated detection antibody specific for Rat ApoH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Human TG in the samples is then determined by comparing the OD of the samples to the standard curve

Guinea Pig IgG (Immunoglobulin G) ELISA Kit Packaging:96 wells The OD value is proportionalThe Guinea Pig (IgG) Immunoglobulin G ELISA Kit measures Immunoglobulin G in Guinea pig samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is