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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SIAL2
The OD value is proportional to the concentration of Rat VCAM-1/CD106
Then Streptavidin-HRP is added and binds to the Biotinylated EDIL3 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated ATP2A3 antibody
Rat BID (Bh3 Interacting Domain Death Agonist) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat (BID) Bh3 Interacting Domain Death Agonist ELISA Kit measures BH3 interacting domain death agonist in Rat samples. The plate has been pre coated with Rat Bid antibody. Bid present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Bid Antibody is added and binds to Bid in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Bid antibody. After incubation unbound Streptavidin HRP is
