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The assay plate has been pre-coated with mouse anti-Mouse CD6 monoclonal antibody
a horseradish peroxidase conjugated mouse anti-Human CD84 Antibody is added to the wells and binds to CD84 in the sample
The concentration of Human GAL4 in the samples is then determined by comparing the OD of the samples to the standard curve
coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus
Human CLDN1 (Claudin 1) ELISA Kit Packaging:96 wells The assay plate has beenThe Human (CLDN1) Claudin 1 ELISA Kit measures Claudin 1 in samples. The plate has been pre coated with Human CLDN1 antibody. CLDN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CLDN1 Antibody is added and binds to CLDN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CLDN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
