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And then biotinylated Canine IL21 Antibody is added and binds to IL21 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated ASRGL1 antibody
it binds with Anti-IFNAR2 bound to precoated antigen
Calculate the concentration of CD28 in the sample by plotting standard curve
Guinea pig HHIP (Hedgehog Interacting Protein) ELISA Kit Packaging:48 wells And then biotinylated Canine IL21The Guinea pig HHIP (Hedgehog Interacting Protein) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig HHIP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig HHIP and Avidin Horseradish Peroxidase (HRP) conjugate are added
