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The Mouse Klotho (Klotho) ELISA kit uses the Sandwich-ELISA principle
The concentration of Human IL28B in the samples is then determined by comparing the OD of the samples to the standard curve
a horseradish peroxidase conjugated mouse anti-Human DOPA Decarboxylase Antibody is added to the wells and binds to DOPA Decarboxylase in the sample
The concentration of Human IL12 in the samples is then determined by comparing the OD of the samples to the standard curve
Human IL-28A ELISA Kit Packaging:96 wells The Mouse Klotho (Klotho) ELISAThe Human IL 28A ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IL28A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IL28A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
