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Calculate the concentration of GPER1 in the sample by plotting standard curve
The OD value is proportional to the concentration of Rat CVL
Then Streptavidin-HRP is added and binds to the Biotinylated SPSB4 antibody
And then biotinylated Human SRI Antibody is added and binds to SRI in the sample
Mouse PGF2Alpha (Prostaglandin F2 Alpha) ELISA Kit PI1 Calculate the concentration of GPER1The Mouse PGF2Alpha (Prostaglandin F2 Alpha) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Mouse PGF2Alpha. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Mouse PGF2Alpha. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
