Mouse EPX (Eosinophil Peroxidase) ELISA Kit (Small Sample) chemokine (C-C motif) ligand 6 Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MCP3

and the degraded protein and PCR inhibitors are removed by the specific washing solution

The concentration of Human BCAM in the samples is then determined by comparing the OD of the samples to the standard curve

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of progesterone present

Mouse EPX (Eosinophil Peroxidase) ELISA Kit (Small Sample) chemokine (C-C motif) ligand 6 Standards or samples are addedThe Mouse EPX (Eosinophil Peroxidase) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse EPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is