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When the sample containing Fractalkine is added to the plate
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G
The concentration of Canine Pg in the samples is then determined by comparing the OD of the samples to the standard curve
coli expression system and the target gene encoding Leu80-Leu235 is expressed
Canine OCLN (Occludin) ELISA Kit Glioblastoma-derived T-cell suppressor factor When the sample containing FractalkineThe Canine (OCLN) Occludin ELISA Kit measures Occludin in Canine samples. The plate has been pre coated with Canine OCLN antibody. OCLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine OCLN Antibody is added and binds to OCLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OCLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
