Human 5HT (5-hydroxytryptamine) ELISA Kit IL6Q Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse TNF-Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Rat MYO in the samples is then determined by comparing the OD of the samples to the standard curve

FST present in the sample is added and binds to antibodies coated on the wells

Akt1 Rabbit Polyclonal Antibody (F201)

Human 5HT (5-hydroxytryptamine) ELISA Kit IL6Q Then a biotinylated detection antibodyThe Human (5HT) 5 hydroxytryptamine ELISA Kit measures 5 Hydroxytryptamine in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added