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Substrate solution is then added and color develops in proportion to the amount of Human GCLM
Then Streptavidin-HRP is added and binds to the Biotinylated SVIL antibody
Only those wells that contain Human TGF-Beta2
a horseradish peroxidase conjugated mouse anti-Mouse EpCAM Antibody is added to the wells and binds to EpCAM in the sample
Human NEO1 (Neogenin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human NEO1 (Neogenin) ELISA Kit measures NEO1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NEO1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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