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SLC38A5 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Mouse RARa
only those wells that contain Mouse Irisin
a capture antibody-ANGPTL7-biotin-detection antibody complex was formed
Human SEMA5A (Semaphorin-5A) ELISA Kit Packaging:96 wells SLC38A5 present in the sampleThe Human SEMA5A (Semaphorin 5A) ELISA Kit measures SEMA5A in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SEMA5A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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