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NEUROD2 present in the sample is added and binds to antibodies coated on the wells
You can calculate the concentration of Rat IgG in the samples by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RLN
Then Streptavidin-HRP is added and binds to the Biotinylated IL26 antibody
Human PKP4 (Plakophilin 4) ELISA Kit Matrilysin NEUROD2 present in the sampleThe Human (PKP4) Plakophilin 4 ELISA Kit measures Plakophilin 4 in samples. The plate has been pre coated with Human PKP4 antibody. PKP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PKP4 Antibody is added and binds to PKP4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PKP4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
