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Then a biotinylated detection antibody specific for LIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human BK in the sample or standard competes with a fixed amount of Human BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BK
The concentration of GATA4 in the sample was calculated by drawing a standard curve
Calculate the concentration of TK2 in the sample by plotting standard curve
Rat DEFB4 (Beta-defensin 4) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat DEFB4 (Beta defensin 4) ELISA Kit measures Defb4 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Defb4 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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