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Then Streptavidin-HRP is added and binds to the Biotinylated CER1 antibody
Then a biotinylated detection antibody specific for Mouse ABeta1-42 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human RANκL
Substrate solution is then added and color develops in proportion to the amount of Human CD200R1
Human GREM1 (Gremlin 1) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human (GREM1) Gremlin 1 ELISA Kit measures Gremlin 1 in samples. The plate has been pre coated with Human GREM1 antibody. GREM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GREM1 Antibody is added and binds to GREM1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GREM1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
