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The assay plate has been pre-coated with mouse anti-Mouse IL-6R monoclonal antibody
And then biotinylated Mouse ATG4D Antibody is added and binds to ATG4D in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated PHF7 antibody
RFTN1 present in the sample is added and binds to antibodies coated on the wells
Human C5A (Complement Component 5a) ELISA Kit PBSF The assay plate has beenThe Human C5A (Complement Component 5a) ELISA Kit measures C5a in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C5a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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