Human SPARC/ON (Osteonectin) ELISA Kit UGB Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated TFAM antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMR1

The concentration of Human ICAM2 in the samples is then determined by comparing the OD of the samples to the standard curve

The OD value is proportional to the concentration of Mouse CKMB

Human SPARC/ON (Osteonectin) ELISA Kit UGB Then Streptavidin-HRP is added andThe Human SPARC ON (Osteonectin) ELISA Kit measures ON in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ON antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.