Canine CCL1 (Chemokine C-C-Motif Ligand 1) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human mTOR

Then a biotinylated detection antibody specific for Mouse CTLA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then Streptavidin-HRP is added and binds to the Biotinylated CA7 antibody

And then biotinylated Mouse SORD Antibody is added and binds to SORD in the sample

Canine CCL1 (Chemokine C-C-Motif Ligand 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Canine CCL1 (Chemokine C C Motif Ligand 1) ELISA Kit measures CCL1 in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCL1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated