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Only those wells that contain Rat NGF
Then Streptavidin-HRP is added and binds to the Biotinylated DLG4 antibody
You can calculate the concentration of Human Cyr61 in the samples by comparing the OD of the samples to the standard curve
ERLIN2 present in the sample is added and binds to antibodies coated on the wells
Human NFAT5 (Nuclear Factor of Activated T-cells 5) ELISA Kit Packaging:96 wells Only those wells that containThe Human (NFAT5) Nuclear Factor of Activated T cells 5 ELISA Kit measures Nuclear Factor of Activated T Cells 5 in samples. The plate has been pre coated with Human NFAT5 antibody. NFAT5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NFAT5 Antibody is added and binds to NFAT5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NFAT5 antibody. After incubation unbound
