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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Pg are added to the micro ELISA plate wells
a horseradish peroxidase conjugated mouse anti-Human Beta-2 microglobulin Antibody is added to the wells and binds to Beta-2 microglobulin in the sample
Then a biotinylated detection antibody specific for Human BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
TRPV6 present in the sample is added and binds to antibodies coated on the wells
Human REG3GAMMA (RegeneRating Islet Derived Protein 3 Gamma) ELISA Kit BNSP Samples (or Standards) and HorseradishThe Human REG3GAMMA (RegeneRating Islet Derived Protein 3 Gamma) ELISA Kit measures REG3Gamma in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti REG3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
