Rat NGF (Nerve Growth Factor) ELISA Kit Packaging:48 wells And then biotinylated Human CELA3B

And then biotinylated Human CELA3B Antibody is added and binds to CELA3B in the sample

And then biotinylated Human FZD7 Antibody is added and binds to FZD7 in the sample

only those wells that contain Rat MMP10

a capture antibody-Shh-biotin-detection antibody complex was formed

Rat NGF (Nerve Growth Factor) ELISA Kit Packaging:48 wells And then biotinylated Human CELA3BThe Rat NGF (Nerve Growth Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well