Sheep EPO (Erythropoietin) ELISA Kit Packaging:96 wells The optical density (OD) is

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 42 nm

Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Substrate solution is then added and color develops in proportion to the amount of Rat TNNI2

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2

Sheep EPO (Erythropoietin) ELISA Kit Packaging:96 wells The optical density (OD) isThe Sheep (EPO) Erythropoietin ELISA Kit measures Erythropoietin in Sheep samples. The plate has been pre coated with Sheep EPO antibody. EPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep EPO Antibody is added and binds to EPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.