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Then Streptavidin-HRP is added and binds to the Biotinylated CNTF antibody
The concentration of Rat BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Porcine IL1A
The Human (FGF3) Fibroblast Growth Factor 3 ELISA Kit measures Fibroblast Growth Factor 3 in samples
Rat HYP (Hydroxyproline) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat (HYP) Hydroxyproline ELISA Kit measures hydroxyproline in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
