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The SIGLEC10 (Sialic Acid Binding Ig Like Lectin 10) ELISA Kit is a Sandwich enzyme immunoassay
The concentration of Mouse IL5Ra in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human TARC in the samples is then determined by comparing the OD of the samples to the standard curve
Rat IL-3 (Interleukin 3) ELISA Kit Packaging:96 wells The SIGLEC10 (Sialic Acid BindingThe Rat (IL 3) Interleukin 3 ELISA Kit measures Interleukin 3 in Rat samples. The plate has been pre coated with Rat IL 3 antibody. IL 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat IL 3 Antibody is added and binds to IL 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL 3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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