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Substrate solution is then added and color develops in proportion to the amount of Human EFHD2
Substrate solution is then added and color develops in proportion to the amount of Rat ANG-1
The concentration of Mouse SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human ASGR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Guinea pig CD97 (Cluster of Differentiation 97) ELISA Kit PADGEM Substrate solution is then addedThe Guinea pig CD97 (Cluster of Differentiation 97) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig CD97. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig CD97 and Avidin Horseradish Peroxidase (HRP) conjugate are added
