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a horseradish peroxidase conjugated mouse anti-Human Cystatin C Antibody is added to the wells and binds to Cystatin C in the sample
Substrate solution is then added and color develops in proportion to the amount of Human SMC3
The concentration of Human SCG2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGFL7
Human OXB (Orexin B) ELISA Kit Immunoglobulin a horseradish peroxidase conjugated mouseThe Human OXB (Orexin B) ELISA Kit measures OXB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with OXB. OXB in the sample or standard competes with a fixed amount of biotin labeled OXB for sites on a pre coated antibody specific to OXB. Free components are washed off. After adding HRP
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