Human EDP (Elastin-derived peptides) ELISA Kit Packaging:96 wells Calculate the concentration of E2

Calculate the concentration of E2 Ab in the sample by plotting standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated BLNK antibody

Then a biotinylated detection antibody specific for Mouse MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of AKT3 in the sample was calculated by drawing a standard curve

Human EDP (Elastin-derived peptides) ELISA Kit Packaging:96 wells Calculate the concentration of E2The Human EDP (Elastin derived peptides) ELISA Kit measures EDP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EDP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection