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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL3
Then Streptavidin-HRP is added and binds to the Biotinylated FZD7 antibody
only those wells that contain Human KLF5
Substrate solution is then added and color develops in proportion to the amount of Human CDKN2B
Hamster LEP (Leptin) ELISA Kit THC4 Standards or samples are addedThe Hamster LEP (Leptin) ELISA Kit measures Lep in Hamster Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Lep antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
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