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Substrate solution is then added and color develops in proportion to the amount of Mouse GKN1
Mouse SST in the sample or standard competes with a fixed amount of Mouse SST on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse SST
And then biotinylated Rat GYS2 Antibody is added and binds to GYS2 in the sample
The plate has been pre-coated with Human HSPB3 antibody
Rabbit TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit CD120b Substrate solution is then addedThe Rabbit TIMP 1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit measures TIMP 1 in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TIMP 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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