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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A4
Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human BMP8B Antibody is added and binds to BMP8B in the sample
The OD value is proportional to the concentration of Rat TNNI3/cTn-I
Human CD244 (Natural Killer Cell Receptor 2B4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (CD244) Natural Killer Cell Receptor 2B4 ELISA Kit measures Natural Killer Cell Receptor 2B4 in samples. The plate has been pre coated with Human CD244 antibody. CD244 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD244 Antibody is added and binds to CD244 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CD244 antibody. After incubation unbound Streptavidin
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