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The color development is stopped with the addition of 50uL stop solution
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RESP18
PCX present in the sample is added and binds to antibodies coated on the wells
The Human PI3 (Peptidase Inhibitor 3
Canine OPN (Osteopontin) ELISA Kit Packaging:96 wells The color development is stoppedThe Canine OPN (Osteopontin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Canine OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine OPN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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