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After a washing step that removes unbound materials
The plate has been pre-coated with Mouse ELANE antibody
The concentration of SPD in the sample was calculated by drawing a standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CHI3L1 antibody
Rabbit VLA4 (Very Late Appearing Antigen 4) ELISA Kit Packaging:96 wells After a washing step thatThe Rabbit VLA4 (Very Late Appearing Antigen 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit VLA4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit VLA4 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
