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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C8g
The concentration of Mouse CTLA4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human FGF21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human NRP2
Goat IL-4 (Interleukin 4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Goat (IL 4) Interleukin 4 ELISA Kit measures Interleukin 4 in Goat samples. The plate has been pre coated with Goat IL4 antibody. IL4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Goat IL4 Antibody is added and binds to IL4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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