Mouse LEP (Leptin) ELISA Kit RCM3 And then biotinylated Mouse FNDC5

And then biotinylated Mouse FNDC5 Antibody is added and binds to FNDC5 in the sample

Substrate solution is then added and color develops in proportion to the amount of Human HTN3

Then a biotinylated detection antibody specific for Human RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The OD value is proportional to the concentration of Human CD74

Mouse LEP (Leptin) ELISA Kit RCM3 And then biotinylated Mouse FNDC5The Mouse (LEP) Leptin ELISA Kit measures Leptin in Mouse samples. The plate has been pre coated with Mouse LEPTIN antibody. LEPTIN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LEPTIN Antibody is added and binds to LEPTIN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPTIN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.