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Excess conjugate and unbound sample or standard are washed away
Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol
You can calculate the concentration of Human Aβ1-42 in the samples by comparing the OD of the samples to the standard curve
This Endothelin 1 ELISA kit uses the Sandwich-ELISA principle
Rat Clusterin ELISA Packaging:96 wells Excess conjugate and unbound sampleThe Rat Clusterin ELISA is an enzyme immunoassay for the quantitative determination of Clusterin in rat serum and rat urine. In the Rat Clusterin ELISA kit, standards and samples are incubated in microtitration wells pre coated with polyclonal anti rat clusterin antibody. After 60 minutes incubation followed by washing, biotin labelled polyclonal anti rat clusterin antibody is added and incubated with the captured clusterin for 60 minutes. After
