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Substrate solution is then added and color develops in proportion to the amount of Rat NT-3
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ST2
the remaining conjugate is allowed to react with the substrate solution (TMB)
CPN2 present in the sample is added and binds to antibodies coated on the wells
Rat FXIIA (Coagulation Factor XIIa) ELISA Kit CLGN Substrate solution is then addedThe Rat FXIIA (Coagulation Factor XIIa) ELISA Kit measures F12a in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Fa antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
