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the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm
The PCR product is blunt-ended and can be directly linked to the blunt-ended cloning vector
The OD value is proportional to the concentration of Human IL-6
and the Human HE4 standard plate wells that pre-coated using protein-related techniques are provided separately
Mouse MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit CEA the enzyme-substrate reaction is terminatedThis Monocyte Chemotactic Protein 1 ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse MCP 1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP 1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
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