Human GKN1 (Gastrokine 1) ELISA Kit Packaging:96 wells Recombinant Mouse Trefoil Factor 3

Recombinant Mouse Trefoil Factor 3 is produced by our Mammalian expression system and the target gene encoding Ala23-Phe81 is expressed with a 6His tag at the C-terminus

Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The sCD130 (Soluble CD130) ELISA Kit is a Sandwich enzyme immunoassay

The concentration of Rat LCT in the samples is then determined by comparing the OD of the samples to the standard curve

Human GKN1 (Gastrokine 1) ELISA Kit Packaging:96 wells Recombinant Mouse Trefoil Factor 3The Human (GKN1) Gastrokine 1 ELISA Kit measures Gastrokine 1 in samples. The plate has been pre coated with Human GKN1 antibody. GKN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GKN1 Antibody is added and binds to GKN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GKN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.