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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LAMa1
The assay plate has been pre-coated with mouse anti-Human ASGR1 monoclonal antibody
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IL4
Substrate solution is then added and color develops in proportion to the amount of Human MIA3
Human PKP1 (Plakophilin 1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (PKP1) Plakophilin 1 ELISA Kit measures Plakophilin 1 in samples. The plate has been pre coated with Human PKP1 antibody. PKP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PKP1 Antibody is added and binds to PKP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PKP1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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