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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BFSP1
Then Streptavidin-HRP is added and binds to the Biotinylated S100A8 antibody
only those wells that contain Rat VIM
and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated
Human SEMA5B (Semaphorin 5B) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (SEMA5B) Semaphorin 5B ELISA Kit measures Semaphorin 5B in samples. The plate has been pre coated with Human SEMA5B antibody. SEMA5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA5B Antibody is added and binds to SEMA5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA5B antibody. After incubation unbound Streptavidin HRP is washed away during a
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