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Substrate solution is then added and color develops in proportion to the amount of Human PRDX2
You can calculate the concentration of Porcine C4BPAlpha in the samples by comparing the OD of the samples to the standard curve
and the color intensity is directly proportional to the amount of Human NBL1 present
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATG7
Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat PDK 4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDK 4 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
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