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Only those wells that contain Mouse TSH
You can calculate the concentration of Human VEGFR-2/KDR in the samples by comparing the OD of the samples to the standard curve
CFHR3 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTN
Human DHFR (Dihydrofolate Reductase) ELISA Kit Packaging:96 wells Only those wells that containThe Human (DHFR) Dihydrofolate Reductase ELISA Kit measures Dihydrofolate reductase in samples. The plate has been pre coated with Human DHFR antibody. DHFR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DHFR Antibody is added and binds to DHFR in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DHFR antibody. After incubation unbound Streptavidin HRP is washed away
