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This FETUB (Fetuin B) ELISA Kit uses the sandwich-ELISA principle
Mouse PGF2Alpha in the sample or standard competes with a fixed amount of Mouse PGF2Alpha on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PGF2Alpha
You can calculate the concentration of Rat SELE in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated IL33 antibody
Rabbit OCLN (Occludin) ELISA Kit Packaging:96 wells This FETUB (Fetuin B) ELISAThe Rabbit OCLN (Occludin) ELISA Kit measures OCLN in Rabbit Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OCLN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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